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Regression of concentration of methylprednisolone on steady-state mRNA levels of TNF-α, IL-1β, and IL-6 in U937 cells primed with LPS. U937 cells (2 × 106/ml) were primed with LPS and exposed to graded concentrations of methylprednisolone for 4 to 6 hs. The results are expressed as the ratio of the mRNA for a particular cytokine to mRNA for β-actin.
