Table 12.
IMGT nomenclature, Eu positions and IMGT motif of engineered Fc variants involved in the knock out CH2 84.4 glycosylation (Effector #8).
| IMGT Engineered Variant Name | IMGT Engineered Variant Definition | IMGT Amino Acid Changes on IGHG CH Domain (Eu Numbering between Parentheses) | Amino Acid Changes with the Eu Positions | Motif Identifiable in Gene and Domain with Positions According to the IMGT Unique Numbering and with Eu Positions between Parentheses | Property and Function |
|---|---|---|---|---|---|
| G1v29 |
CH2
A84.4 |
CH2 N84.4 > A (297) |
N297A |
IGHG1 CH2 83–86 REEQYNSTYRVV > REEQYASTYRVV |
ADCC reduction. Reduces FcγR binding [132] |
| G1v30 |
CH2
G84.4 |
CH2 N84.4 > G(297) |
N297G |
IGHG1 CH2 83–86 REEQYNSTYRVV > REEQYGSTYRVV |
ADCC reduction. Reduces FcγR binding [132] |
| G1v36 |
CH2
Q84.4 |
CH2 N84.4 > Q (297) |
N297Q |
IGHG1 CH2 83–86 REEQYNSTYRVV > REEQYQSTYRVV |
ADCC reduction. Reduces FcγR binding |
| G4v36 |
CH2
Q84.4 |
CH2 N84.4 > Q (297) |
N297Q |
IGHG4 CH2 83–86 REEQFNSTYRVV > REEQFQSTYRVV |
ADCC reduction. Reduces FcγR binding |
|
Canis lupus familiaris
G2v29 |
CH2
A84.4 |
CH2 N84.4 > A (297) |
N297A |
IGHG1 CH2 83–86 REEQFNGTYRVV > REEQFAGTYRVV |
ADCC reduction. Reduces FcγR binding |