Table 15.
IMGT nomenclature, Eu positions and IMGT motif of engineered Fc variants involved in the formation of additional bridge stabilizing CH2 in the absence of N84.4 (297) glycosylation (Structure #11).
| IMGT Engineered Variant Name | IMGT Engineered Variant Definition | IMGT Amino Acid Changes on IGHG CH Domain (Eu Numbering between Parentheses) | Amino Acid Changes with the Eu Positions | Motif Identifiable in Gene and Domain with Positions According to the IMGT Unique Numbering and with Eu Positions between Parentheses | Property and Function |
|---|---|---|---|---|---|
| G1v54 |
CH2
C83, C85 |
CH2 R83 > C (292), V85 > C (302) |
R292C, V302C |
IGHG1 CH2 83–86 REEQYNSTYRVV > CEEQYASTYRCV (v29) CEEQYGSTYRCV (v30) CEEQYQSTYRCV (v36) |
Stabilizes CH2 in the absence of N84.4 (297) glycosylation |
| G1v54-29 |
CH2
C83, A84.4, C85 |
CH2 R83 > C (292), N84.4 > A(297) V85 > C (302) |
R292C, N297A V302C |
IGHG1 CH2 83–86 REEQYNSTYRVV > CEEQYASTYRCV |
Stabilizes CH2 in the absence of N84.4 (297) glycosylation |
| G1v54-30 |
CH2
C83, G84.4, C85 |
CH2 R83 > C (292), N84.4 > G (297) V85 > C (302) |
R292C, N297G V302C |
IGHG1 CH2 83–86 REEQYNSTYRVV > CEEQYGSTYRCV |
Stabilizes CH2 in the absence of N84.4 (297) glycosylation |
| G1v54-36 |
CH2
C83, Q84.4, C85 |
CH2 R83 > C (292), N84.4 > Q (297) V85 > C (302) |
R292C, N297Q V302C |
IGHG1 CH2 83–86 REEQYNSTYRVV > CEEQYQSTYRCV |
Stabilizes CH2 in the absence of N84.4 (297) glycosylation |