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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: Pflugers Arch. 2017 Nov 13;470(2):339–353. doi: 10.1007/s00424-017-2084-x

Fig. 5. High Na+ high Cl− diet decreases ClC-K2/b activity on the basolateral membrane of collecting duct intercalated cells.

Fig. 5

Representative macroscopic currents in response to voltage steps from −90 to + 60 mV (shown in inset) from the holding potential of − 60 mV (a) and averaged current-voltage (I–V) relations (b) in intercalated cells from mice kept on regular diet (0.3% Na+, 0.5% Cl−: RNa+ RCl−, black) and high NaCl diet (1.6% Na+, 2.4% Cl−: HNa+ HCl−, gray). c Representative continuous current traces from cell-attached patches monitoring basolateral 10 pS ClC-K2/b single channel activity in collecting duct intercalated cells for the regular (left) and high NaCl (right) conditions. Patches were clamped to −Vp = − 60 mV. “C” denotes non-conducting closed state. Summary graph of changes in open probability (d), number of active channels within a patch (e), and unitary current amplitude (f) of ClC-K2/b in intercalated cells from mice on regular diet and high NaCl diet. *Significant difference versus regular diet