Table 6.

Associations^a of BA concentrations with rectal tissue microbiome alpha diversity at baseline (N = 65)

BA, ng/mL	Alpha diversity metricsb
	Shannon	Observed ASVs	Faith PD
	Rs; P	Rs; P	Rs; P
Chenodeoxycholic acid	−0.20; 0.12	−0.10; 0.43	−0.08; 0.53
Cholic acid	−0.06; 0.64	0.09; 0.47	0.10; 0.43
Deoxycholic acid	−0.29; 0.02	−0.39; 0.002	−0.22; 0.08
Glycochenodeoxycholic acid	−0.14; 0.28	−0.06; 0.65	−0.20; 0.11
Glycocholic acid	−0.19; 0.13	−0.16; 0.22	−0.25; 0.05
Glycodeoxycholic acid	−0.30; 0.02	−0.33; 0.01	−0.29; 0.02
Glycolithocholic acid	0.02; 0.88	0.07; 0.61	−0.06; 0.67
Glycoursodeoxycholic acid	−0.23; 0.07	−0.30; 0.02	−0.31; 0.01
Lithocholic acid	−0.01; 0.92	−0.001; 0.99	0.06; 0.66
Taurochenodeoxycholic acid	−0.20; 0.12	−0.15; 0.23	−0.28; 0.02
Taurocholic acid	−0.19; 0.13	−0.18; 0.16	−0.31; 0.01
Taurodeoxycholic acid	−0.33; 0.01	−0.39; 0.002	−0.39; 0.002
Ursodeoxycholic acid	−0.24; 0.06	−0.31; 0.01	−0.20; 0.12
Summary scores
Total BAs	−0.26; 0.04	−0.21; 0.10	−0.26; 0.04
Primary BAsc	−0.16; 0.20	−0.03; 0.81	−0.02; 0.90
Secondary BAsd	−0.29; 0.02	−0.39; 0.001	−0.24; 0.05

ASV, amplicon sequence variant; BA, bile acid; PD, phylogenetic diversity.

^aSpearman correlations were adjusted for age and sex.

^bAlpha diversity mean and SD: Shannon Index 5.40 (1.07), observed ASVs 198.68 (67.72), and Faith's PD 21.54 (4.96).

^cPrimary BAs = log₂ of the sum of chenodeoxycholic acid, cholic acid, glycocholic acid, glycochenodeoxycholic acid, taurocholic acid, and taurochenodeoxycholic acid.

^dSecondary BAs = log₂ of the sum of deoxycholic acid, lithocholic acid, glycodeoxycholic acid, glycolithocholic acid, and taurodeoxycholic acid.