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. Author manuscript; available in PMC: 2023 Oct 27.
Published in final edited form as: Cell. 2022 Oct 27;185(22):4153–4169.e19. doi: 10.1016/j.cell.2022.09.033

Figure 2. SYK deficiency in microglia exacerbates Aβ pathology and leads to behavior and memory defects.

Figure 2.

(A) Representative images of Aβ1–42 staining in 6- and 9-month-old Sykfl/fl-5×FAD and SykiΔMG-5×FAD mice.

(B) Percentage of Aβ1–42+ area in the cortex of 6- and 9-month-old mice of each genotype. Data points represent the average of three technical repeats (one experiment; n = 7–8 mice/genotype).

(C) Representative confocal images of methoxy-X04 and Lamp1+ dystrophic neurites in the cortex of 9-month-old Sykfl/fl-5×FAD and SykiΔMG-5×FAD mice.

(D) Volumes of dystrophic neurites per plaque in 6- and 9-month-old mice of each genotype. Data points represent the average of two technical repeats (one experiment; n = 5–9 mice/genotype).

(E-G) Morris water maze tests on 6- and 9-month-old Sykfl/fl and SykiΔMG mice with or without 5×FAD transgenes. F) Latency time to submerged platform; (G) time in target quadrant without submerged platform.

(H-J) Elevated plus maze test on 6- and 9-month-old Sykfl/fl and SykiΔMG mice with or without 5×FAD transgenes. (I) Percentage of entries into open arms; (J) percentage of travelled distance in open arms.

Sample size of behavioral tests: 6-month-old Sykfl/fl (n=6), SykiΔMG (n=7), Sykfl/fl-5×FAD (n=12) and SykiΔMG-5×FAD (n=11); 9-month-old Sykfl/fl (n=7), SykiΔMG (n=6), Sykfl/fl-5×FAD (n=6) and SykiΔMG-5×FAD (n=9). *, P < 0.05; **, P < 0.01; ***, P < 0.001 by two-way ANOVA with Sidak’s multiple comparisons test (B, D, F, I and J), or one-way ANOVA with Tukey’s multiple comparisons test (G). Data are presented as mean ± SEM.

See also Figure S2 in the supplemental information.