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. Author manuscript; available in PMC: 2022 Nov 1.
Published in final edited form as: J Med Chem. 2021 Sep 20;64(19):14876–14886. doi: 10.1021/acs.jmedchem.1c01426

Figure 2.

Figure 2.

Bioconjugation of rIL2 with TLHE (3). (A) Schematic of the reaction of rIL2 with TLHE to generate TLHE-rIL2. (B) MALDI-TOF MS spectrum of rIL2. (C) MALDI-TOF MS spectrum of the TLHE-rIL2 conjugation reaction mixture. (D) SDS-PAGE analysis for the rIL2 and TLHE-rIL2 conjugation reaction. Lane 1: Prestained marker; Lane 2: unlabeled rIL2; Lane 3: TLHE-rIL2 conjugation reaction mixture. (E) MS/MS spectrum of the m/z 383.67 double charged ion of the tryptic peptide PC(+57.02)SSSTK, containing the carbamidomethyl modification at the cysteine 3 residue. (F) MS/MS spectrum of the m/z 516.25 triple charged ion of the tryptic peptide PC(+837.43)SSSTK, containing the TLHE modification at the cysteine 3 residue.