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. 2022 Nov 2;25(12):105479. doi: 10.1016/j.isci.2022.105479

Figure 1.

Figure 1

Extended immunization did not enhance RBD specific antibody production in mice

(A)The conventional or extended RBD vaccine immunization strategy. Black arrows represent 50 μg RBD injection. Red arrows represent mice sacrifice on the seventh day after the last immunization. Tail vein peripheral blood was collected from mice on the 10th day after each immunization. Peripheral blood was collected on the seventh day before the first immunization as the negative control.

(B) RBD-specific IgG antibody titers were tested by ELISA in mice sera taken 10 days following each injection. (N = 3).

(C) RBD binding IgG antibody titers were tested by ELISA in mice sera taken 10 days following the fourth and sixth immunization. Points represent individual mice. Data were presented as mean ± SEM.

(D) RBD-specific IgG1 antibody titers were tested by ELISA in mice sera taken 10 days following each vaccination. (N = 3).

(E) RBD-specific IgG2a antibody titers were tested by ELISA in mice sera taken 10 days following each immunization. (N = 3). ∗p< 0.05. ns represented non-significant. ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, ∗∗∗∗p< 0.0001. ns represented non-significant.