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. 2022 Nov 2;25(12):105479. doi: 10.1016/j.isci.2022.105479

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© 2022.

This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).

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Extended immunization suppressed the formation of the germinal center

(A) The percentages of Fas⁺ GL-7⁺ B cells (gated CD19⁺ B cells) from the splenocytes on the seventh day after the last immunization were detected by flow cytometry.

(B) On the seventh day after the last immunization, the frozen tissues of the mouse spleens were stained with PNA (green) and B220 (red). The scale bar represented 75 μm, and the pictures were analyzed by ImageJ software.

(C) The percentages of PD-1⁺ CXCR5⁺ Tfh cells from the splenocytes were detected by flow cytometry and shown in gated CD4⁺T cells. Points represent individual mice in (A) and (C). Data were presented as mean ± SEM ∗p< 0.05, ∗∗∗p< 0.001. ns represents non-significant.