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. 2022 Nov 2;25(12):105479. doi: 10.1016/j.isci.2022.105479

Figure 5.

Figure 5

Extended immunization inhibited the activation of CD4+T cell immune responses

The expression of CD69 (A) and CD137 (B) (gated on CD4+T cells) were detected by flow cytometry on the seventh day after the last immunization.

(C) The ratio of Tn (CD62L+ CD44−), Te (CD62L− CD44−), Tem (CD62L− CD44+) and Tcm (CD62L+ CD44+) of CD4+T cells were detected by flow cytometry on day 7 after the last immunization.

(D) The expression of PD-1 and LAG-3 (gated on CD4+T cells) were detected by flow cytometry on day 7 after the last immunization.

(E) The ratio of Treg (gated on CD4+) was detected by flow cytometry on the seventh day after the last immunization. IL-10 (F) in the immunized serum were detected on the seventh day after the last immunization. Data were presented as mean ± SEM ∗p< 0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, ∗∗∗∗p< 0.0001. ns represented non-significant.