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. 2022 Nov 2;25(12):105479. doi: 10.1016/j.isci.2022.105479

Figure 6.

Figure 6

Extended immunization inhibited CD8+ T cell-mediated immune response

IL-2 (A), IFN-γ (B) and TNF-α (C) in the immunized serum were detected on the seventh day after the last immunization. The expression of CD69 (D) and CD137 (E) in splenocytes (gated on CD8+T cells) were respectively detected by flow cytometry after 10 μg/mL RBD or HBV peptide stimulation for 24 h, conventional media was used as negative control.

(F) The ratio of Tn (CD62L+ CD44−), Te (CD62L− CD44−), Tem (CD62L− CD44+) and Tcm (CD62L+ CD44+) of CD8+T cells on day 7 after the last immunization.

(G) The expression of PD-1 and LAG-3 (gated on CD8+T cells) were detected on day 7 after the last immunization. Data were presented as mean ± (SEM). ∗p<0.05, ∗∗p< 0.01, ∗∗∗p< 0.001, ∗∗∗∗p< 0.0001. ns represented non-significant.