REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
APC anti-mouse CD19	BioLegend	Cat#152409; AB_2629838
PE anti-mouse CD138	BioLegend	Cat#142503; AB_10915989
FITC anti-mouse/rat/human CD27	BioLegend	Cat#124207; AB_1236463
PE/Cyanine7 anti-mouse CD4	BioLegend	Cat#100421; AB_312706
APC/Cyanine7 anti-mouse CD185 (CXCR5)	BioLegend	Cat#145525; AB_2566798
PE anti-mouse CD279 (PD-1)	BioLegend	Cat#135205; AB_1877232
FITC anti-mouse/human GL7 Antigen	BioLegend	Cat#144603; AB_2561696
PerCP/Cyanine5.5 anti-mouse CD95 (Fas)	BioLegend	Cat#152609; AB_2632904
Alexa Fluor® 647 anti-mouse/human CD45R/B220 Antibody	BioLegend	Cat#103229; AB_492875
Alexa Fluor 700 anti-mouse CD3	BioLegend	Cat#100216; AB_493697
PE anti-mouse CD8a	BioLegend	Cat#162303; AB_2894434
Brilliant Violet 605™ anti-mouse CD69	BioLegend	Cat#104529;AB_11203710
APC anti-mouse CD137	BioLegend	Cat#106109; AB_2564296
APC anti-mouse CD279	BioLegend	Cat#109111; AB_10613470
PE anti-mouse CD223	BioLegend	Cat#125208; AB_2133343
Brilliant Violet 605™ anti-mouse/human CD44	BioLegend	Cat#103047; AB_2562451
Brilliant Violet 421™ anti-mouse CD62L	BioLegend	Cat#104435; AB_10900082
Alexa Fluor® 488 anti-mouse FOXP3	BioLegend	Cat#136803; AB_10946412
HRP-conjugated Goat Anti-Mouse IgG H&L secondary antibody	Abcam	Cat#ab6789; AB_955439
HRP-conjugated Goat Anti-Mouse IgG1 H&L	Bethyl	Cat#A90-105P; AB_67150
HRP-conjugated Goat Anti-Mouse IgG2a H&L	Bethyl	Cat#A90-107P; AB_67155
Goat anti-mouse IgG-ALP	MabTech	Cat#3310-4; AB_2890180
Bacterial and virus strains
SARS-COV-2-S	GenBank	QVE75681.1
SARS-CoV-2-SB.1.617.2	GenBank	EPI_ISL_4299998
SARS-COV-2-SOmicron	GenBank	EPI_ISL_7263803
Biological samples
RBD recombination protein	Stored in the lab	N/A
Chemicals, peptides, and recombinant proteins
RBD-his protein	Sinobiological	Cat#40592-V05H
RBD-mfc protein	Sinobiological	Cat#40592-V05H
ACE2-his protein	Sinobiological	Cat#10108-H08H
Critical commercial assays
LIVE/DEADTM Fixable Dead Cell Stain Kit	Invitrogen	Cat#L34976
Mouse and Rat cytokine Assays kit	Bio-RAD	Cat#10014905
Experimental models: Cell lines
HEK 293T	ATCC	N/A
293F cells	ATCC	N/A
Experimental models: Organisms/strains
Balb/c mice; females	Experimental Animal Center of Chongqing Medical University	N/A
Software and algorithms
Graphpad Prism 8	Graphpad Prism 8	N/A
Flow jo version 10.5.2.	Flow jo version 10.5.2.	N/A
Other
6-well cell culture plates	Thermo Fisher	Cat#140675
Corning CellBIND Surface 100 mm Culture Dish	Corning	Cat#3296
Bio-plex mouse cytokines detection kit	Bio-rad	Cat#M60000007A
ELISPOT plates	Thermo Fisher	Cat#AB2384B