TABLE 1.
Primer sequences used in this study
Primer | Sequence | Comments |
---|---|---|
P1 | 5′CTC GGA TCC ATG AAA TTT AAG TCA TTG ATT ACA ACA ACA TTA GCA TTA GG 3′ | Nucleotides 71–111 of map; BamHI site underlined |
P2 | 5′ CTC GGT ACC TTA AAA TTT AAT TTC AAT GTC TAC TTT TTT AAT GTC 3′ | Nucleotides 2139–2105 of map; KpnI site underlined |
P3 | 5′CTC GGA TCC GCA GCT AAG CCA TTA GAT AAA TCA TCA AGT TCG TTA CAC C3′ | Nucleotides 91–130 of eap-N; BamHI site underlined |
P4 | 5′CTC GGA TCC GCA GCT AAG CCA TTA GAT AAA TCA TCA AGT TTA TTA CAT C3′ | Nucleotides 91–130 of eap-W; BamHI site underlined |
P5 | 5′CTC GGA TCC AAA ATA GAG AAA GTC TGG CTA TAA TTA AGT TGC 3′ | Nucleotides 3–35 of map; BamHI site underlined |