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. 2022 Oct 4;13(21):3042–3051. doi: 10.1111/1759-7714.14657

FIGURE 3.

FIGURE 3

PTPL1 knockdown facilitates NSCLC cell proliferation in vitro. (a) CCK8 assays of control and PTPL1 knockdown cells. Cells (2 × 103 were seeded in 96‐well plates, and the OD values were measured per 24 h for 4 days. *p < 0.05 compared with control group. (b) Cell proliferation was analyzed by immunofluorescence staining for EdU. (c) Cell proliferation was analyzed by colony formation assay. Knockdown of PTPL1 expression significantly promoted cell proliferation in NSCLC cell. Scale bar = 20 μm or (EdU) 100 μm (colony). Data shown are mean ± SD (n = 5). (*p < 0.05, **p < 0.01, ***p < 0.001). CCK8, Cell counting kit‐8 assay; EdU, ethynyldeoxyuridine; NSCLC, non‐small cell lung cancer; OD, optical density; PTPL1, protein‐tyrosine phosphatase‐L1