FIGURE 6.

PTPL1 inhibits growth of lung cancer in vitro through downregulation of YAP1. (a) Cell counting kit‐8 (CCK8) assays of control and YAP1 knockdown cells. *p < 0.05 compared with control group. (b) Cell proliferation was analyzed by immunofluorescence staining for EdU. (c) Cell proliferation was analyzed by colony formation assay. Knockdown of YAP1 expression alleviates the inhibition of cell proliferation by PTPL1 in NSCLC cells. Scale bar = 20 μm or (EdU) 100 μm (colony). Data shown are mean ± SD (n = 5). (*p < 0.05, **p < 0.01, ***p < 0.001). EdU, ethynyldeoxyuridine; NSCLC, non‐small cell lung cancer; PTPL1, protein‐tyrosine phosphatase‐L1; shmock, negative control; shPTPL1, short hairpin RNA against PTPL1; shmock, negative control; shPTPL1, short hairpin RNA against PTPL1; shYAP1, short hairpin RNA against YAP1; YAP1, yes‐associated protein