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. 2022 Sep 13;13(21):2992–3000. doi: 10.1111/1759-7714.14648

FIGURE 1.

FIGURE 1

KLF2 is a direct target of miR‐92a‐3p. (a) The potential regulators of KLF2 predicted by starBase v.3.0. (b) The correlation between candidate miRNAs and KLF2 expression. (c) The relative miR‐92a‐3p expression levels in breast cell lines were determined by qRT‐PCR. (d) The mRNA expression levels of KLF2 in miR‐92a‐3p overexpressed MCF‐7 cells and T47D cells (left), and in miR‐92a‐3p‐depleted CAL‐51 cells and MDA‐231 cells (right) detected by qRT‐PCR. (e) The protein levels of KLF2 in miR‐92a‐3p overexpressed MCF‐7 cells and T47D cells, and in miR‐92a‐3p‐depleted CAL‐51 cells and MDA‐231 cells were detected by western blot. (f) The predicted binding of miR‐92a‐3p with KLF2 3′ UTR. (g) Enrichment of KLF2 in MCF‐7 cells after pull‐down assay with biotinylated miR‐92a‐3p. (h) The regulation of miR‐92a‐3p on KLF2 was detected by a dual‐luciferase reporter assay. *p < 0.05