FIGURE 4.

KLF2 repressed BIRC5 expression transcriptionally. (a) The correlation expression levels of KLF2 with BIRC5 and CCND1. (b) The potential binding site of KLF2 on the BIRC5 promoter region was predicted by JASPAR. (c) ChIP analysis for investing the interaction between KLF2 and BIRC5 promoter, including endogenous interaction (left) and exogenous interaction (right). (d) Dual‐luciferase reporter analysis of BIRC5 promoter activity in KLF2‐transfected 293FT cells. The expression level of BIRC5 in KLF2‐overexpressed MDA‐231 and CAL‐51 cells, as well as in KLF2‐depleted MCF‐7 and T47D cells, was detected by qRT‐PCR (e) and western blot (f). *p < 0.05