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. 2022 Oct 28;58:102519. doi: 10.1016/j.redox.2022.102519

Fig. 7.

Fig. 7

S-sulfhydration at cysteine 607 prevented Drp1 and VDAC1 interaction and regulated ISO-induced mitochondrial dysfunction. A, Western blots showing Drp1 and VDAC1 interactions in the indicated hearts of mice after 4 weeks' vehicle or ISO administration. B, Western blots showing the interactions between Drp1 WT and VDAC1 or Drp1 C607A and VDAC1 in the adult cardiomyocytes incubated with or without ISO (1 μmol/L, 48 h) and NaHS (30 μmol/L, 48 h). C, Representative linescan confocal images and summarized data showing the laser-induced permanent loss of Δψm in individual mitochondrion. n = 547–900 mitochondria in 31–37 cells from 4 to 5 rats in each group. D-E, Effects of ISO (1 μmol/L, 48 h) on ATP levels (D), and cell death (E) in adult cardiomyocytes with or without overexpression of Drp1 WT or C607A, or pretreatment of VBIT-4 (10 μmol/L, 48 h). n = 3–4. F, Representative fluorescence images and quantification of mt-SoNar or mt-cpYFP in adult cardiomyocytes expressed Drp1 WT or C607A with or without ISO (1 μmol/L, 48 h), NaHS (30 μmol/L, 48 h) or VBIT-4 (10 μmol/L, 48 h). n = 29–34 cells from 3 rats in each group. Scale bar = 20 μm. G, Western blots images and summarized data showing effects of ISO on Bcl-2, Bax and cleaved caspase 9 in adult cardiomyocytes and with overexpression of Drp1 WT or C607A or pretreatment of VBIT-4. n = 4. *: p < 0.05, **: p < 0.01.