Fig. 8.

Algorithm of integrated interdisciplinary diagnostics in astrocytoma patients. CDKN2A cyclin-dependent kinase inhibitor 2A homozygous deletion, CNV copy number variation, EGFR-amp epidermal growth factor receptor amplification, FISH fluorescence in situ hybridization, HE hematoxylin and eosin staining, IDH isocitrate dehydrogenase, IHC immunohistochemistry, MGMT O6-methylguanine-DNA methyltransferase, MS-PCR methylation-specific PCR, NGS next-generation sequencing, PCR polymerase chain reaction, STB stereotactic biopsy, TERT telomerase reverse transcriptase promoter pathogenic variants, mt mutant, wt wildtype, + 7/− 10 combination of chromosome 7 gain and 10 loss. *Not elsewhere classified (NEC); NEC diagnosis is facilitated by the use of layered integrated reports; integrated diagnosis combined tissue-based histological and molecular diagnosis