Table 3.

Change of diagnosis based on NGS findings and new WHO CNS5 2021 classification

N	Prior diagnosis (WHO 2016)	% of tumor cells	Gene	Change	Variant	Final diagnosis (WHO 2021)	Change of diagnosis
1	AA	85	TERT	NC_000005.9:g.1295228G>Aa	Pathogenic	GBM	Yes
2	AA	80	TERT	NC_000005.9:g.1295228G>Aa	Pathogenic	GBM	Yes
3	AA	100	TERT	NC_000005.9:g.1295228G>Aa	Pathogenic	GBM	Yes

AA anaplastic astrocytoma, IDH-wildtype, WHO CNS5 2021 the fifth edition of the WHO Classification of Tumors of the Central Nervous System, GBM glioblastoma, IDH-wildtype, N patient number, NGS next-generation sequencing, TERT human telomerase reverse transcriptase, WHO World Health Organization

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