Fig. 5. Quantification of oxidized complex lipids in blood plasma lipid extracts of lean non-diabetic (LND), obese non-diabetic (OND) and obese with type 2 diabetes (OT2D) individuals.

In silico prediction of sample-specific epilipidome, its identification using stDDA followed by PRM-based targeted quantification allowed to annotate and quantify oxidized lipids with significant differences between three phenotypes (a). Heatmaps illustrate lean (LND), obese (OND) and T2D obese (OT2D) specific epilipidomics signatures for oxPC (b), oxTG (c) and oxCE (d) lipids with significantly different levels (ANOVA p < 0.05). Source data are provided as a Source Data file. Based on the identified phenotype-specific epilipidomes, the endocrine functionality was proposed for complex lipids esterified octadecanoids, which was altered upon obesity and type 2 diabetes via enrichment of lipid peroxidation (LPO)-derived eicosanoids (e).