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Real-time live confocal microscopy during normothermic machine perfusion. (a–c) Representative RTCM images stained with SYTO 16 (green), PI (red), WGA (pink) and merge; score 0 = highly viable cells (a), score 1 = number of viable and dead cells equals (b), score 2 = highly non-viable cells (c). Scale bars represent 100 μm. Semiquantitative scoring for the transplanted (d) and discarded livers (e). Results are expressed as mean ± SD.
