Table 2.
Donor demographics and preservation characteristics.
| Donor characteristics | Overall cohort (N = 50) | Transplanted (N = 35) | Discarded (N = 15) | p-value |
|---|---|---|---|---|
| Age [y, median (min–max)] | 59 (15–85) | 62 (26–85) | 56 (15–84) | 0.7576 |
| Sex ratio [N (%)] | ||||
| Male | 26 (52%) | 16 (46%) | 10 (67%) | |
| Female | 24 (48%) | 19 (54%) | 5 (33%) | 0.1742 |
| BMI [kg m−2, median (IQR)] | 27.0 (24.0–30.0) | 26.0 (24.0–29.0) | 28.0 (23.0–31.0) | 0.5018 |
| Cause of death [N (%)] | ||||
| CVA | 29 (58%) | 21 (60%) | 8 (53%) | |
| Circulatory | 7 (14%) | 4 (11%) | 3 (20%) | |
| Trauma | 11 (22%) | 7 (20%) | 4 (27%) | |
| Other | 3 (6%) | 3 (8%) | 0 (0%) | 0.5460 |
| Donor type [N (%)] | ||||
| DBD | 37 (74%) | 29 (83%) | 8 (53%) | |
| DCD | 13 (26%) | 6 (17%) | 7 (74%) | 0.0292 |
| ECD [N (%)] | 43 (86%) | 28 (80%) | 15 (100%) | |
| DRI [median (IQR)] | 1.89 (1.63–2.24) | 1.78 (1.61–2.07) | 2.22 (1.90–2.38) | 0.1471 |
| Preservation and NMP | ||||
| CIT [min, median (IQR)] | 366 (299–447) | 349 (299–433) | 372 (294–483) | 0.7518 |
| Functional WITa [DCD; min, median (IQR)] | 25.50 (21.75–28.75) | 26.00 (23.50–33.25) | 25.00 (20.75–28.75) | 0.8860 |
| Duration of NMP [min, median (IQR)] | 882 (574–1277) | 824 (603–1177) | 1231 (471–1309) | 0.7762 |
| Total preservation time [min, median (IQR)] | 1240 (983–1576) | 1170 (971–1549) | 1466 (1106–1686) | 0.3629 |
| Bile volume [mL, median (IQR)] | 82.0 (7.9–191.8) | 185.4 (39.3–348.5 | 81.9 (18.3–160.0) | 0.4385 |
| Lactate clearance [< 2.5 mmol L−1 at 2 h NMP, N (%)] | 44 (88%) | 31 (89%) | 13 (87%) | |
| Perfusate lactate [mmol L−1, median (IQR)] | ||||
| 15 min | 6.44 (4.22–9.32) | 5.55 (3.89–7.33) | 7.66 (5.63–8.74) | 0.0682 |
| 1 h | 1.61 (0.94–3.91) | 1.22 (0.67–2.44) | 3.55 (1.11–5.99) | 0.0702 |
| 2 h | 1.22 (0.78–3.5) | 1.11 (0.61–1.89) | 1.22 (0.80–3.91) | 0.2643 |
| 6 h | 0.89 (0.44–1.55) | 0.78 (0.39–1.22) | 2.44 (1.19–2.89) | 0.0086 |
| 12 h | 0.67 (0.36–3.05) | 0.56 (0.39–1.16) | 2.61 (0.42–3.77) | 0.2751 |
| 20 h | 0.78 (0.44–2.00) | 0.72 (0.47–1.19) | 2.05 (1.25–4.27) | 0.1337 |
| Perfusate AST [U L−1, median (IQR)] | ||||
| 15 min | 1631 (730–4097) | 1238 (663–2101) | 6075 (3655–11246) | 0.0005 |
| 1 h | 1853 (1051–5194) | 1482 (729–3136) | 7308 (3416–14431) | 0.0012 |
| 2 h | 1918 (991–4430) | 1644 (757–3090) | 7372 (1941–12606) | 0.0070 |
| 6 h | 2369 (1203–6721) | 1820 (1055–4725) | 10392 (4297–20842) | 0.0010 |
| 12 h | 5098 (1838–10234) | 2547 (1498–6764) | 16274 (9006–24193) | 0.0009 |
| 20 h | 7571 (3585–17566) | 5507 (2592–8872) | 16939 (8745–28965) | 0.0797 |
| Perfusate ALT [U L−1, median (IQR)] | ||||
| 15 min | 1389 (527–3352) | 814 (450–1762) | 4672 (2337–11017) | 0.0013 |
| 1 h | 1514 (626–4024) | 1065 (549–2067) | 6639 (3100–8828) | 0.0020 |
| 2 h | 1561 (631–4416) | 1234 (541–2459) | 6702 (2382–8535) | 0.0037 |
| 6 h | 1804 (741–5238) | 1272 (644–2730) | 8448 (3287–14077) | 0.0015 |
| 12 h | 2828 (1071–6667) | 1832 (938–3735) | 8910 (4796–15045) | 0.0025 |
| 20 h | 4196 (1429–8378) | 3136 (1208–4441) | 9224 (4425–16903) | 0.0295 |
| Perfusate LDH [U L−1, median (IQR)] | ||||
| 15 min | 2664 (1243–6960) | 2053 (1125–3338) | 13017 (3517–23729) | 0.0010 |
| 1 h | 2968 (1671–10891) | 2619 (1523–4826) | 12957 (6295–26347) | 0.0014 |
| 2 h | 3401 (1556–8640) | 2943 (1521–5820) | 10475 (4433–21975) | 0.0122 |
| 6 h | 3946 (2095–11115) | 3139 (2019–7062) | 11908 (5148–25884) | 0.0078 |
| 12 h | 6589 (3394–15502) | 4331 (2949–10528) | 22103 (9525–37958) | 0.0052 |
| 20 h | 8830 (5270–20269) | 6622 (3613–13922) | 20917 (8407–27371) | 0.0392 |
a
Functional warm ischemia time is applied for DCD donors and defined as the time from a mean arterial pressure below 50 mmHg or an arterial saturation of <80% until the start of cold perfusion.