Table 4.
Mitochondrial respiration in the OXPHOS state and corresponding flux control ratios.
| Transplanted | pre | 1 h | 6–9 h | 9–15 h | 15–24 h | post |
|---|---|---|---|---|---|---|
| F-pathway [pmol s−1 mg−1] | 12.54 (8.88–14.82) | 11.18 (7.26–16.83) | 10.83 (7.23–14.60) | 11.48 (6.71–13.28) | 10.48 (7.97–15.20) | 10.72 (6.07–13.77) |
| N-pathway [pmol s−1 mg−1] | 4.81 (2.99–6.67) | 4.91 (3.66–7.43) | 7.19 (3.75–9.10) | 6.06 (4.37–9.76) | 6.82 (6.19–8.13) | 6.14 (3.44–7.49) |
| S-pathway [pmol s−1 mg−1] | 40.23 (33.57–52.15) | 41.04 (36.83–51.96) | 44.39 (35.38–54.13) | 44.47 (35.9–57.39) | 41.17 (32.74–52.35) | 35.89 (28.07–51.70) |
| F FCR | 0.30 (0.25–0.34) | 0.27 (0.21–0.31) | 0.27 (0.21–0.31) | 0.24 (0.19–0.31) | 0.27 (0.21–0.32) | 0.27 (0.22–0.35) |
| N FCR | 0.13 (0.10–0.15) | 0.14 (0.10–0.17) | 0.13 (0.10–0.18) | 0.15 (0.10–0.18) | 0.18 (0.14–0.21) | 0.15 (0.09–0.19) |
| S FCR | 1.00 (0.98–1.02) | 1.00 (0.99–1.02) | 1.00 (0.98–1.04) | 1.01 (0.98–1.03) | 1.02 (1.01–1.05) | 1.00 (0.98–0.13) |
| Discarded | pre | 1 h | 6–9 h | 9–15 h | 15–24 h |
|---|---|---|---|---|---|
| F-pathway [pmol s−1 mg−1] | 13.81 (8.09–19.24) | 7.68 (5.71–10.27) | 7.78 (6.10–9.51) | 9.08 (5.93–10.76) | 12.42 (10.26–14.58) |
| N-pathway [pmol s−1 mg−1] | 5.50 (2.82–8.76) | 3.20 (2.11–5.54) | 4.37 (2.71–6.31) | 6.18 (3.54–9.13) | 6.68 (5.78–8.60) |
| S-pathway [pmol s−1 mg−1] | 48.26 (35.15–59.53) | 41.05 (30.97–55.36) | 42.74 (27.01–43.31) | 44.44 (37.29–47.98) | 45.56 (38.02–48.92) |
| F FCR | 0.29 (0.24–0.38) | 0.21 (0.14–0.27) | 0.19 (0.14–0.30) | 0.21 (0.14–0.27) | 0.27 (0.25–0.29) |
| N FCR | 0.12 (0.09–0.17) | 0.10 (0.08–0.11) | 0.11 (0.08–0.17) | 0.16 (0.08–0.19) | 0.15 (0.14–0.16 |
| S FCR | 0.99 (0.96–1.03) | 0.98 (0.97–1.02) | 1.00 (0.97–1.00) | 0.99 (0.98–1.00) | 0.99 (0.95–1.01) |
Respiration in the OXPHOS state with saturating ADP concentrations (5 mM) normalized for wet tissue mass. Octanoylcarnitine (0.5 mM) and malate (0.1 mM) were added as substrates for fatty acid oxidation (F-pathway); pyruvate (5 mM), malate (2 mM) and glutamate (10 mM) as substrates for the NADH-linked N-pathway; or rotenone (0.5 μM) and succinate (10 mM) for the S-pathway. Relative contributions of the three electron transfer pathways are expressed as flux control ratios (FCR). The FCR of the F-, N-, and S-pathways were calculated relative to the maximum OXPHOS capacity reached after addition of substrates and ADP for all three pathways. Results are shown as median (interquartile range).