REAGENT or RESOURCE	SOURCE	IDENTIFIER
Antibodies
Mouse Monoclonal Anti-polyHistidine antibody	Sigma	Cat#H1029
Rabbit anti-mouse peroxidase conjugate	Sigma	Cat#A9044
Bacterial and virus strains
E. Coli DH5 alpha	Thermofischer	Cat#18265017
AcMNPV (Bac10:KO1629,Δv-cath/chiA-LoxP:DsRed)	Kolesnikova et al. (2022)	N/A
E. coli OP50	CGC	https://cgc.umn.edu/strain/OP50
Chemicals, peptides, and recombinant proteins
GMAP-1-8X Histidine	This study	N/A
cOmplete™ His tag purification resin	Merck	Cat# 5893682001
Superdex® 200 10/300 GL	Cytiva	Cat# GE17-5175-01
PIP Micro-Strips	Echelon Biosciences	Cat#P-6001
PIP Micro-Strips	Echelon Biosciences	Cat#P-6002
NBD-PC	Merck	Cat# 810131P
NBD-PS	Merck	Cat# 810198C
Cas9-NLS Purified Protein	IDT-DNA	Cat# 1081058
Alt-R® CRISPR-Cas9 tracrRNA, 5 nmol	IDT	Cat#1072532
Critical commercial assays
Vivaspin 2 centrifugal concentrators	Merck	Cat# Z614211
Experimental models: Cell lines
Adapted Sf9 cells	Thermofisher	Cat# B82501
Grace Medium supplemented with 10% FCS	Thermofisher	Cat# 11595030
Adapted High Five cells	Thermofisher	Cat# B85502
Express Five medium	Thermofisher	Cat#10486025
Experimental models: Organisms/strains
bus-5 (br19) X	CGC	DC19
bus-8 (e2883) X.	CGC	CB6177
bus-17(br2) X.	CGC	CB7431
acs-20 (e3031) IV.	CGC	CB7198
agmo-1(e3016) III.	CGC	LC144
bli-1(e769) II.	CGC	CB769
pld-1(ok2222) II.	CGC	RD1737
gmap-1(ulb13)	This study	OQ192
gmap-1(ulb13); bli-1(e769)	This study	OQ396
gmap-1(ulb13); pld-1(ok2222)	This study	OQ396
gmap-1(ulb13); Ex[pgmap-1::egfp + ccrfp]	This study	OQ194
gmap-1(ulb13); Ex[pgmap-1::gmap-1(cDNA)::sl2 gfp + ccrfp]	This study	OQ195
gmap-1(ulb13); Ex[pgmap-1::gmap-1(cDNA)::egfp + ccrfp]	This study	OQ196
gmap-1(ulb13); Ex[pagmo-1::gmap-1(cDNA)::sl2 gfp + ccrfp]	This study	OQ197
gmap-1(ulb13); Ex[pC05 × 107.1::egfp + ccrfp]	This study	OQ198
gmap-1(ulb13) X.; Ex[pAgmo-1::mEGFP + pAgmo-1::gmap-1::mCherry]	This study	OQ286
Ex[pAgmo-1::mEGFP + pgmap-1::gmap-1::mCherry]	This study	OQ288
bli-1(e769) II.; Ex[pAgmo-1:gmap-1::mCherry + pAgmo-1::mEGFP]	This study	OQ397
frIs7 [nlp-29p::GFP + col-12p::dsRed] IV	CGC	IG274
gmap-1(ulb13) X.; frIs7 [nlp-29p::GFP + col-12p::dsRed] IV.	This study	OQ283
gmap-1(ulb13) X; frIs7 [nlp-29p::GFP + col-12p::dsRed] IV.	This study	OQ284
qxIs722 [dpy-7p::dpy-7::SfGFP (single copy)]	CGC	XW18042
gmap-1(ulb13) X; qxIs722 [dpy-7p::dpy-7::SfGFP (single copy)]	This study	OQ347
cgEx198 [(pJC14) bli-1::GFP + unc-119(+)].	CGC	CH1445
gmap-1(ulb13); cgEx198	This study	OQ398
gmap-1(ulb13); Ex[pgmap-1::gmap-1::sl2 gfp + ccRFP]	This study	OQ399
knockin[ctns-1::wrmScarlet]+ Ex[pgmap-1::gmap-1-GFP-10ng/ul]	This study	OQ449
cas9 knockin [ctns-1::wrmScarlet]	Stigloher lab	PHX5270
gmap-1(ulb13); dpy-10(e928)	This study	OQ428
gmap-1(ulb13); Ex[phsp-16.41::gmap-1::mCherry + ccRFP]	This study	OQ449
Oligonucleotides
pGMAP-1Fow AttB4 GGGGACAACTTTGTATAGAAA AGTTGGTGGCCCATGAAACAGTTGAC	This study	N/A
pGMAP-1Rev AttB1 GGGGACTGCTTTTTTGTACAA ACTTGTCATTAACGCAAGTCGTGTTTC	This study	N/A
pagmo1 Fwd AttB4 GGGGACAACTTTGTATA GAAAAGTTGTTAACTTGGCAAACA GTTGGAAGC	This study	N/A
pagmo1 Rev AttB1 GGGACTGCTTTTTTGTAC AAACTTGGCCTCTTTTCATTTGG TTAAAATTT	This study	N/A
pC05 × 107.1 Fwd AttB4 GGGGACAACTTTGT ATAGAAAAGTTGATACAATTTTTCTTT CCTCC	This study	N/A
pC05 × 107.1 Rev AttB1 GGGACTGCTTTTTTGTACA AACTTGGAAGTTACAAGAACAG TATAGAAAAAG	This study	N/A
GMAP-1 cDNA (stop) Fwd AttB1 GGGGACAAGTTTGTA CAAAAAAGCAGGCTATGACGACCCG AAGAATCGTAC	This study	N/A
GMAP-1 cDNA (stop) Rev AttB2 GGGGACCACTTTGT ACAAGAAAGCTGGGTTTAGTTTGGCTG GGACACCG	This study	N/A
GMAP-1 Fwd + BamH1 ATCTAATTTAGGATCCATGAC GACCCGAAGAATCGTAC	This study	N/A
GMAP-1 Rev + EcoR1 GTTATACCTCTAGAATTCGTT TGGCTGGGACACCGTC	This study	N/A
Translational reporter GMAP-1 Fwd GACTGTAAATCATACGATTTGGTGG	This study	N/A
Translational reporter GMAP-1 Rev TGCCCAGTTCACATCTACCAATATTG	This study	N/A
gmap-1 Ext FWD TGCGTTAATGACGACCCGAAG	This study	N/A
gmap-1 Int FWD CAGAGTGAAGATTGCGCTTCAAAGTTTTAAAAC	This study	N/A
gmap-1 Ext REV AGTTTGGCTGGGACACCGTC	This study	N/A
acs-20(tm3232) Ext FWD ACTACGGAACCAAAGACCCT	This study	N/A
acs-20(tm3232) Int FWD CCTTAGATTTTTGCCCGCTTCTGC	This study	N/A
acs-20(tm3232) Ext REV ACTCGAACAACTGACGCCAT	This study	N/A
pld-1(ok2222) Ext FWD GCCAAGTATTTCCGATTCCACC	This study	N/A
pld-1(ok2222) Int REV CAACAGAGAAAGAAGGATCGGC	This study	N/A
pld-1(ok2222) Ext REV GTAATTCGGTTCCGAGAACACG	This study	N/A
Recombinant DNA
pAC8_MF	Kolesnikova et al. (2022)	Addgene Plasmid #139768
pAC8_MF modified linker for expression of a protein fused to a C-terminal 8 His tag (BamHI-HindIII fragment) GGATCCCGGTCCGAAGCGCGCGGAATTCTCTAGAGAAA ACCTGTACTTCCAAGCGCACCATCACCATCACCATCATC ACTGAACTAGTGCCTGCAGTCTCGACAAGCTT	This study	N/A
Software and algorithms
ImageJ/fiji	Schindelin et al. (2012)	N/A
Tierpsy	Javer et al. (2018)	N/A
Fidabio software suite	Fida Biosystems ApS	N/A
Prism 9.2.0	GraphPad	https://www.graphpad.com/scientific-software/
Illustrator 26.3.1	Adobe	https://www.adobe.com/products/illustrator.html
ApE	Wayne Davis	https://jorgensen.biology.utah.edu/wayned/ape/