TABLE 2.
Overall rankings for A. japonicus reference genes.
| Conditions | References gene | geNorm | NormFinder | BestKeeper | ΔCt | Recommendation | ||||
|---|---|---|---|---|---|---|---|---|---|---|
| Stability | Rank | Stability | Rank | Stability | Rank | Stability | Rank | |||
| Developmental stage | EF1α | 0.256 | 4 | 0.622 | 6 | 0.214 | 1 | 0.828 | 4 | RPL13 |
| TATA | 0.576 | 6 | 0.545 | 4 | 0.719 | 5 | 0.862 | 6 | ||
| TUB | 0.463 | 5 | 0.582 | 5 | 0.757 | 6 | 0.848 | 5 | ||
| GAPDH | 0.854 | 8 | 1.320 | 8 | 0.968 | 7 | 1.378 | 8 | ||
| RPS6 | 0.107 | 1 | 0.054 | 1 | 0.313 | 4 | 0.593 | 1 | RPS6 | |
| RPS3a | 0.129 | 3 | 0.210 | 3 | 0.22 | 2 | 0.631 | 3 | ||
| RPL13 | 0.107 | 1 | 0.054 | 1 | 0.264 | 3 | 0.602 | 2 | ||
| ACTIN | 0.680 | 7 | 0.996 | 7 | 0.973 | 8 | 1.093 | 7 | ||
| Tissue | EF1α | 0.367 | 5 | 0.114 | 1 | 0.5465 | 2 | 0.751 | 5 | ACTIN |
| TATA | 0.469 | 6 | 0.974 | 7 | 1.218 | 8 | 1.063 | 6 | ||
| TUB | 0.669 | 7 | 0.841 | 6 | 0.435 | 1 | 1.158 | 7 | ||
| GAPDH | 0.972 | 8 | 1.85 | 8 | 0.693 | 4 | 1.882 | 8 | ||
| RPS6 | 0.139 | 3 | 0.385 | 3 | 0.894 | 5 | 0.721 | 2 | RPS6 | |
| RPS3a | 0.039 | 1 | 0.500 | 5 | 0.944 | 7 | 0.742 | 3 | ||
| RPL13 | 0.039 | 1 | 0.488 | 4 | 0.929 | 6 | 0.743 | 4 | ||
| ACTIN | 0.318 | 4 | 0.114 | 1 | 0.554 | 3 | 0.716 | 1 | ||
| Sex | EF1α | 0.001 | 1 | 0 | 1 | 0.638 | 5 | 0.422 | 1 | RPL13 |
| TATA | 0.305 | 6 | 0.660 | 6 | 0.941 | 8 | 0.685 | 5 | ||
| TUB | 0.081 | 3 | 0.038 | 3 | 0.553 | 4 | 0.422 | 1 | ||
| GAPDH | 0.593 | 8 | 0.862 | 8 | 0.025 | 2 | 0.878 | 8 | ||
| RPS6 | 0.119 | 4 | 0.038 | 3 | 0.499 | 3 | 0.443 | 4 | EF1α | |
| RPS3a | 0.248 | 5 | 0.581 | 5 | 0.896 | 7 | 0.629 | 6 | ||
| RPL13 | 0.001 | 1 | 0 | 1 | 0.639 | 6 | 0.422 | 1 | ||
| ACTIN | 0.498 | 7 | 0.818 | 7 | 0 | 1 | 0.846 | 7 | ||
| Diapause | EF1α | 0.285 | 5 | 0.343 | 5 | 0.419 | 2 | 0.497 | 5 | ACTIN |
| TATA | 0.196 | 3 | 0.214 | 2 | 0.381 | 1 | 0.437 | 3 | ||
| TUB | 0.349 | 6 | 0.227 | 4 | 0.673 | 6 | 0.482 | 4 | ||
| GAPDH | 0.393 | 7 | 0.376 | 6 | 0.724 | 7 | 0.547 | 7 | ||
| RPS6 | 0.531 | 8 | 0.91 | 8 | 0.837 | 8 | 0.947 | 8 | TATA | |
| RPS3a | 0.117 | 1 | 0.219 | 3 | 0.451 | 4 | 0.416 | 2 | ||
| RPL13 | 0.251 | 4 | 0.432 | 7 | 0.427 | 3 | 0.526 | 6 | ||
| ACTIN | 0.117 | 1 | 0.129 | 1 | 0.487 | 5 | 0.396 | 1 | ||
| All conditions | EF1α | 0.426 | 3 | 0.89 | 7 | 0.693 | 4 | 1.089 | 7 | RPS3a |
| TATA | 0.829 | 7 | 0.598 | 6 | 0.769 | 6 | 1.027 | 6 | ||
| TUB | 0.786 | 6 | 0.462 | 1 | 0.753 | 5 | 0.975 | 4 | ||
| GAPDH | 1.087 | 8 | 1.781 | 8 | 1.303 | 8 | 1.861 | 8 | ||
| RPS6 | 0.722 | 5 | 0.576 | 4 | 0.779 | 7 | 1.004 | 5 | RPL13 | |
| RPS3a | 0.24 | 1 | 0.5 | 2 | 0.662 | 3 | 0.878 | 1 | ||
| RPL13 | 0.24 | 1 | 0.576 | 5 | 0.657 | 2 | 0.903 | 2 | ||
| ACTIN | 0.572 | 4 | 0.56 | 3 | 0.631 | 1 | 0.957 | 3 | ||