Figure 3.

TNFR1^−/− Improves Survival of HF-Activated T Cells

(A) Representative flow scatterplots for donor CD45.2⁺ CD4⁺ T cells isolated either from WT or TNFR1^−/− mice in the blood of CD45.1⁺ mice at 2 days and 1, 2, and 14 weeks and (B) their group quantitation at 2 days and 1, 2, 4, 8, and 14 weeks post-AT. (C) Representative flow scatterplots and (D) group quantitation for donor CD45.2⁺ CD4⁺ T cells in the spleens of CD45.1 recipient mice at 14 weeks. Group quantitation for (E) donor-derived CD45.2⁺ CD4⁺ Bcl-xL⁺ and (F) CD45.2⁺ CD4⁺ CD44^high CD62L⁺ cells at 14 weeks post-AT. The study was repeated 2 times (n = 3-5 in each study), and data from a representative experiment are shown. Data in B were analyzed using repeated measures 2-way analysis of variance, and P values were adjusted using the false discovery rate. Data in D, E, andF were analyzed using the unpaired Student’s t-test. AT = adoptive transfer; WT = wild type; other abbreviations as in Figure 1.