Figure 5.

TNFR1^−/− Promotes Proliferation of HF-Activated T Cells

(A) Representative flow contour plots for HF-activated WT or TNFR1^−/− donor CD45.2⁺ CD4⁺ T cells in the hearts of CD45.1⁺ recipient mice and (B) their group quantitation for the total number of cells (left) and their frequency (right) with respect to total cardiac CD4⁺ T cells at 14 weeks. (C) Representative flow scatterplots showing ki67⁺ cells in CD45.2⁺ CD4⁺ T cells infiltrated into the hearts of CD45.1 recipient mice and (D) their group quantitation (frequency [left] and ki67 MFI [right]) at 14 weeks post-AT. Donor-derived CD45.2⁺ CD4⁺ Ki67⁺ T cells in the (E) blood and (F) spleen of recipient CD45.1 mice at 14 weeks post-AT. Studies were repeated 2 times (n = 2-5 in each), and cumulative data are shown. Ki67 MFI and splenic cell counts are representative from 1 experiment. Data were analyzed using 2-way analysis of variance with P values adjusted using the false discovery rate in BandD (left) and the unpaired Student’s t-test in D (right), E, andF.