Fig. S11.

Total nucleic acid extraction optimization. (a), Comparison of TNA extraction from DBS. Fifty microliters of spiked sheep blood was dried on Whatman 903™ Cards and stored for 24 h at room temperature. TNA were extracted from 3 punches of 6 mm using the RNeasy mini kit (QIAGEN), RNeasy micro kit (QIAGEN) or the NucleoSpin Triprep (Macherey–Nagel). Bars represent mean readout + SD of 4 SHERLOCK replicates, shown as grey circles. (b), Trypanosome TNAs extraction from spiked human buffy coat using Maxwell automated system. Two Maxwell RSC kits (simplyRNA blood kit and Blood DNA kit) and two different input volumes (125 μL and 250 μL) were compared. (c), SHERLOCK assay for 7SLRNA target. Uninfected human blood was spiked with 1000 p/μL followed by dilution series. Buffy coat was obtained by centrifugation and TNAs were purified either with Maxwell automated system (Maxwell RSC Blood DNA kit) or with the manual column-based QIAGEN RNeasy mini kit, with minor modifications (see materials and methods). The data from the Maxwell extraction system is also shown in Fig. 3b.