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. 2022 Oct 14;66:101615. doi: 10.1016/j.molmet.2022.101615

Figure 1.

Figure 1

Long-term activation of mTORC1 with exercise and leucine in SOL. (A) Experimental set-up. Quantification (B–G) and representative images (H) of pRPS6Ser235/236 (B), pRPS6Ser240/244 (C), pS6K1Thr389 (D), γ-isoform of 4E-BP1 (E), 4E-BP1Ser65 (F), and pEEF2Thr56 (G). (I–J) Representative immunoblot (I) and quantification (J) of puromycin incorporation. Bars represent mean, circles represent individual values, error bars represent standard error of mean (SEM). All data is shown as fold change to Sed sal (dashed horizontal line). Panel B–G and J, (Sed sal n = 8), (Sed leu n = 10) (Run0 n = 6) (Run48 n = 6). One-Way ANOVA with Tukey’s multiple comparisons test (panel B–G and J). ∗ p < 0.05; ∗∗ p < 0.01, ∗∗∗p < 0.001, ∗∗∗∗p < 0.001.