ATF4 and downstream gene expression is increased after exercise. (A) Quantification of ATF4. (B) qPCR of AA transporters in SOL. (C) Quantification of LARS. (D) representative immunoblots. Bars represent mean, circles represent individual values, error bars represent standard error of mean (SEM). All data is shown as fold change to Sed sal. Panel A–C, (Sed sal n = 4), (Run0 n = 6) (Run48 n = 4). One-Way ANOVA with Tukey's multiple comparisons test. ∗ p < 0.05; ∗∗ p < 0.01, ∗∗∗p < 0.001.