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. 2022 Sep 24;298(11):102532. doi: 10.1016/j.jbc.2022.102532

Figure 4.

Figure 4

CX3CL1-ICD induces expression of genes for proliferation.A–D, significant overexpression of neuronal differentiation markers ASCL1 and NeuroD1 as well as cell cycle regulators, such as cyclin, Cdk, and PCNA, was observed in CX3CL1-ct/tTa mice, using lysates prepared the same as in Figure 3. Sox2, Sox5, Sox8, and Sox9 were also elevated and shown in (C). B and D, bar graphs show comparative protein expression levels normalized to the loading control. N = 3 independent experiments (∗p < 0.05; ∗∗p < 0.01; ∗∗∗p < 0.001, one-way ANOVA). E and G, the same set of protein levels was compared on the Western blots using protein lysates from N2A cells treated with Tet34 or Tet34s for 24 or 48 h. F and H, bar graphs show protein expression levels normalized to the loading control. N = 3 independent experiments (∗p < 0.05; ∗∗p < 0.01; one-way ANOVA). CX3CL1-ICD, intracellular domain of CX3CL1.