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. 2022 Sep 7;82(21):4044–4057. doi: 10.1158/0008-5472.CAN-21-4443

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©2022 The Authors; Published by the American Association for Cancer Research

This open access article is distributed under the Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International (CC BY-NC-ND 4.0) license.

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Figure 5. Phospho- and total-proteomics reveals DNA repair pathways are activated upon VRK1 knockdown in VRK2-low cells. A, Heat maps showing total-proteomics (left) and phospho–proteomics (right) in VRK2-low and VRK2-high U251MG cells at 5 and 7days after doxycycline. B, Gene Set Enrichment Analysis (GSEA) of 7-day total proteomics data in the doxycycline conditions. C, Volcano plots differential expression analysis of total proteomic data; The x-axis represents log2 fold change and the y-axis represents the FDR [−log10(q value)]; black circles—proteins with greater than ± 2 log-fold change. D, Volcano plots differential expression analysis of phospho–proteomic data; The x-axis represents log2 fold change and the y-axis represents the FDR [−log10(q value)]; black circles—proteins with greater than ± 2 log-fold change. E, Immunoblots of select proteins from U251MG VRK2-low and VRK2-high cells treated with or without doxycycline for 7 days — Phospho- and total proteomics reveals DNA repair pathways are activated upon VRK1 knockdown in VRK2-low cells. A, Heat maps showing total proteomics (left) and phosphoproteomics (right) in VRK2-low and VRK2-high U251MG cells at 5 and 7days after doxycycline. B, Gene set enrichment analysis of 7-day total proteomics data in the doxycycline conditions. C, Volcano plots of differential expression analysis of total proteomic data. The x-axis represents log₂-fold change and the y-axis represents the FDR [−log₁₀(q value)]; black circles, proteins with greater than ± 2 log-fold change. D, Volcano plots of differential expression analysis of phosphoproteomic data. The x-axis represents log₂-fold change and the y-axis represents the FDR [−log₁₀(q value)]; black circles, proteins with greater than ± 2 log-fold change. E, Immunoblots of select proteins from U251MG VRK2-low and VRK2-high cells treated with or without doxycycline for 7 days.