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. 2022 Oct 19;9:1014475. doi: 10.3389/fvets.2022.1014475

Table 1.

List of primers and probes designed for the present study.

Primer Sequence Ref. accession
Real time PCR
PPV-1 Fw ATTCGGGCAGAGATGTAACG MF681710
PPV-1 Rv TATCCCACCCCTCGGTCTAT
PPV-1 Probe FAM-GCCCGATAAAATCCACAAAGA-BHQ1
SOV Fw GGGGAGGACTTGATGCTGTA KX364383.1
SOV Rv AACTTTGCTGCCTCCTTTGA
SOV Probe FAM-CTGAAAGCTGAGAAGGCCAG-BHQ1
PCMV Fw AATGCGTTTTACAACTTCACG KF017583.1
PCMV Rv CTGAGCATGTCCCGCCCTAT
PCMV Probe FAM-CTCTAGCGGCGTCCATCACC-BHQ1
PRCV Fw TCAGCCAATTTTGGTGACAG KY406735.1
PRCV Rv GATCATCCTTTGGCAAGTGG
PRCV Probe FAM-ATGGGAGCAGTGCTAAGCAT-BHQ1
End-point PCR
PRCV Fw AAACACTACTTGTGGTTTTGGTTAT MF462726
PRCV Rv ACAGTCACACCGAACGGAAT
PCMV Fw TGACAGTGAGCAGTCGGAAT KX575707.1
PCMV Rv TCAGGCGTGGATATGTAACG
SOV Fw CTATCGGAACCGAATGAGAC KX364383.1
SOV Rv TGCCAGGAGCCATATTTG