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. 2022 Nov 2;12(11):220203. doi: 10.1098/rsob.220203

Figure 2.

Figure 2.

Mck1 kinase activity is vital for its SPOC function. (a) Serial dilutions of Gal1-yeGFP-KIN4 MCK1 and Gal1-yeGFP-KIN4 mck1Δ cells carrying an empty plasmid (pRS315), pRS315-MCK1 or pRS315-mck1-kinase dead (KD) as indicated. Growth is shown on Gal1-KIN4 repressing (glucose) or inducing (galact.) selection plates after 3 days at 30°C. (b) Percentage of SPOC-deficient phenotypes for the indicated strains carrying an integrated copy of MCK1 and mck1-KD. Average ± s.d. of three independent experiments (n = 100 anaphase cells per strain and experiment) is shown. (c) Serial dilutions of wild-type and Gal1-yeGFP-KIN4 strains carrying the indicated gene deletions on glucose (Gal1 repressing) and galactose (Gal1 inducing) plates. Growth is shown after 3 days at 30°C. (d). SPOC-deficient phenotypes of indicated strains. Graph shows average ± s.d. of three independent experiments (n = 100 cells per strain and experiment). Asterisks in (b) and (d) indicate a significant difference based on the two-tailed Student's t-test, p < 0.05 (*), p < 0.001 (**) and p < 0.0001 (***).