Figure 5.

Flow cytometry analyzed the apoptosis of A549 cells received irradiation or not and treated with DMEM, CAF₀-CM, CAF₈-CM. (A) CAFs exhibited an inhibitory effect on A549 cells apoptosis, which showed more obvious in radiation-activated CAFs. In the unirradiated group, the CAF₈-CM subgroup displayed more normal cells with less viable apoptotic cells. And when A549 received 6Gy irradiation, the number of the viable apoptotic cells in DMEM subgroup was twice as many as that in the CAF₈-CM subgroup, which indicated the protective value of CAFs for irradiated A549; (B) A quantitative statistical analysis of the apoptotic cells in the different groups was carried out, and the bar graphs visualized the differences in apoptosis. (T-test, data are presented as the mean ± S.D. *P < 0.05, **P < 0.01, ***P < 0.001).