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. 2022 Oct 19;10:944828. doi: 10.3389/fbioe.2022.944828

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Copyright © 2022 Manon, Evrard, Maistriaux, Fievé, Heller, Magnin, Boisson, Kadlub, Schubert, Lengelé, Behets and Cornu.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Comparison of HP (top) and HFL (bottom including zoom in) by Masson’s trichrome staining (A), Sirius red staining (B), immunohistochemistry for Type 1 collagen (C), electron microscopy (D) and collagen quantification (N = 4 donors × three samples from each donor leading to n = 12 specimens analyzed for each tissue) (E). C: cambial layer, F: fibrous layer, L: longitudinal layer, T: transverse layer, HP: human periosteum, HFL: human fascia lata. Black scale bars: 100 µm. Yellow scale bars: 500 µm. Red scale bars: 5 µm. **** p < 0.0001.