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Comparison of the HP and HFL by multiplex immunohistochemistry with tyramide signal amplification for CD90, CD73 and CD105 with merged signals (A) or separate signals (B). Blue: Hoechst, Green: CD90, Yellow: CD73, Red: CD105. White arrows show the simultaneous presence of the 3 signals on the same location. C: cambial layer, F: fibrous layer, L: longitudinal layer, T: transverse layer. White scale bars: 100 µm.
