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. 2022 Aug 25;9:uhac185. doi: 10.1093/hr/uhac185

Figure 3.

Figure 3

Pollen viability and pollen morphology of SlHB8 gene knockout and overexpression lines. a Scanning electron micrographs of pollen grains from wild-type, SlHB8 gene knockout and SlHB8 overexpression lines under control of the 35 s and 2A11 promoters. BM: binucleate microspore stage; MI: microspore mother cell stage; MP: mature pollen stage; MUM: middle uninucleate microspore; Tds: tetrad stage. From the top to the bottom, the scale bar indicated 20 μm, 2 μm, 1 μm, respectively. b Pollen viability after TTC staining. Scale bar = 100 μm. c, d Percentage of pollen viability in wild-type and SlHB8 transgenic lines. e, f Expression levels of SlHB8 in the transgenic tomato lines p35s::SlHB8Ris and p2A11::SlHB8Ris during anther development. Ubi was used as reference gene. Expression level in MI stage flower bud was used as control. The error bars denote SE; ***P < 0.001 (Student’s t-test; compared with the WT).