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. 2022 Aug 25;9:uhac185. doi: 10.1093/hr/uhac185

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© The Author(s) 2022. Published by Oxford University Press on behalf of Nanjing Agricultural University.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Histocytological observation of pollen development in the wild-type, SlHB8 gene knockout and SlHB8 overexpression lines under control of the 35 s promoter. DAPI staining (a) and semi-thin section comparison (b) of anther and pollen development between the wild-type, SlHB8 gene knockout and 35 s-L2 lines. APG: abnormal pollen grain; BM: binucleate microspore stage; MI: microspore mother cell stage; MP: mature pollen stage; MSP: microspore; MUM: middle uninucleate microspore stage; PG: pollen grain; T: tapetum; Td: tetrad; Tds: tetrad stage. The white arrows indicate the nuclei (a). The scale bar indicated 20 μm (a) and 100 μm (b).