Figure 8.

Validation of SlHB8 downstream target genes via yeast one-hybrid, EMSA and dual-luciferase assays. a Validation of SlHB8 binding with the four selected DAP-seq fragments using yeast one-hybrid assay. AD-empty indicates the control yeast strain transformed with the empty pGADT7 vector without SlHB8. b EMSA validation of the binding motifs in four selected DAP-seq fragments. The corresponding motif are listed beside the band. The detected bands are indicated with black arrows. c Validation of SlHB8 activation on five selected promoters using a dual-luciferase assay. The empty effector was used as control (set as 1). The ratio is presented as the mean ± SE (n = 3).