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. 2022 Sep 15;37(11):2635–2645. doi: 10.1093/humrep/deac204

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© The Author(s) 2022. Published by Oxford University Press on behalf of European Society of Human Reproduction and Embryology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com

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Figure 2. — Granulosa cell mitotic index as a measure of follicle development rate in adult Amh^+/+ and Amh^−/− mice. (A) transitioning follicles (Amh^+/+: n = 29, Amh^−/−: n = 19), (B) primary follicle (Amh^+/+: n = 17, Amh^−/−: n = 35) and (C) secondary follicles (Amh^+/+: n = 14, Amh^−/−: n = 41) showed no significant difference between the genotypes (Student’s t-test). (D) Example of PCNA immunohistochemical staining (black arrow) and unstained (white arrow) granulosa cells in a secondary follicle from an Amh^−/− mouse. Data are expressed as mean ± SEM Scale bar: 50 µm. AMH, anti-Müllerian hormone; PCNA, proliferating cell nuclear antigen.