TABLE 1.

Recombinant polypeptide fragments of Emb and oligonucleotide primers used for PCR amplification

Fragment	Amino acid residues	kDaa	Position of oligonucleotide primerb
			5′	3′ (complement)
Emb-A	503–963	48.0	(NdeI) 1754–1770	3135–3117 (BamHI)
Emb-B	928–139	48.5	(NdeI) 3029–3045	4414–4399 (BamHI)
Emb-C	50–530	51.8	(EcoRI) 349–412	1837–1821 (BamHI)
Emb-D	50–661	65.6	(EcoRI) 349–412	2230–2212 (BamHI)

^aFor the Emb-derived portion of the fused protein only. Recombinant fragments C and D included a six-residue N-terminal sequence encoded by the plasmid. 

^bPCR primers were designed to include the nucleotide sequence of the emb gene as shown. Each primer included a unique restriction enzyme site (indicated in parentheses). Additional nucleotides were added to maintain the reading frame (5′ primer) or to introduce a stop codon (3′ primer).