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. 2022 Oct 19;13:981817. doi: 10.3389/fphar.2022.981817

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Copyright © 2022 del Río, Ruiz-Pino, Prados, Fiebich, Tena-Sempere and Muñoz.

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CBD reduces liver fibrosis induced by CCl4. (A) Representative images of collagen staining in the liver using picrosirius red dye (upper panel) or immunostaining with Tnc (bottom panel) from control, CBD, CCl4-vehicle, and CCl4 + CBD mice. Scale bars represent 100 and 50 μm, respectively. (B) Quantification of positive collagen content (left panel) and Tnc staining (right panel), expressed as a percentage of the total liver area. Values are expressed as mean ± SEM (n = 6 animals per group). ***p < 0.001 vs. control group; ^### p < 0.001 vs. CCl4 group (ANOVA followed by Tukey’s test).