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. 2022 Sep 19;41(21):e111338. doi: 10.15252/embj.2022111338

Figure EV2. Validation of the CRISPRi screen results.

Figure EV2

  1. qRT–PCR results showing the targeted genes (IRF6, MYBL2 and ZBTB7B) were efficiently knocked down by the inducible CRISPRi system using the gRNAs selected from the CRISPRi gRNA library.
  2. Representative EdU staining images of non‐targeting gRNA control and IRF6 or MYBL2 knock‐down experiments.
  3. Quantification of the percentage of EdU+ cells in each of three parental organoid lines used with non‐targeting control, IRF6 knock‐down and MYBL2 knock‐down. n = 1,649, 1,705, 3,548 cells were scored for NT controls. n = 2,517, 950, and 1,313 cells were scored for IRF6 gRNAs. n = 1,098 and 1,306 cells were scored for MYBL2 gRNAs.
  4. qRT–PCR results showing ARID5B was not knocked down by the inducible CRISPRi system using the gRNAs selected from the CRISPRi gRNA library.

Data information: Error bars: mean ± SEM. Statistical analysis was using the two‐tailed paired t‐test. P‐values are reported as follows: *P < 0.05, **P < 0.01, ***P < 0.001 and n.s. non‐significant. N = 3 organoid lines (biological replicates) used for each panel.

Source data are available online for this figure.