Figure EV2. SFI1 depletion alters centriolar Centrin position but not CP110.

• A
  Representative confocal images of mitotic control and SFI1‐depleted RPE1‐1 cells stained for Centrin (Cetn2/3, green) and CP110 (magenta). Scale bar: 5 μm.
• B
  CP110 (magenta) and Centrin (green) relative integrated intensities from a plot profile across the 2 centrioles in control and SFI1‐depleted cells (siSFI1#A and siSFI1#A′ correspond to two different siRNAs, see material and methods). Average ± SD, N, statistical analysis: siCT (area under the curve) = CP110: 1 ± 0.3, Cetn2/3: = 0.99 ± 0.5. siSFI1#A (area under the curve) = CP110: 0.9 ± 0.3, Cetn2/3: 0.4 ± 0.1. siSFI#A′ (area under the curve) = CP110: 1 ± 0.3, Cetn2/3: 0.5 ± 0.2. N = 60 cells from three independent experiments. Unpaired t‐test (***P < 0.0001).