Figure 3.

Characterization of AME-E-like cells transcriptome

(A) PCA of transcriptomes during in vitro differentiation: naive hPSCs to TE-like, partially primed hPSCs to AME-E-like, and primed hPSCs to AME-L-like cells.

(B) Heatmap showing clustering analysis of genes differentially expressed between any two time points of differentiation to AME-E-like cells.

(C) Gene ontology analysis of differentially expressed genes between AME-E-like cells and hPSCs.

(D) Immunofluorescence for organelles markers in AME-E-like cells and hPSCs.