Figure 4.

Molecular signatures of trophectoderm, early amnion, and late amnion in primates

(A) Clustering analysis of all genes differentially expressed between any two cell types among hsTE, hsAME-E, and cyAME-L3.

(B) Comparison of gene expression in embryo cell populations and their putative in vitro counterparts. The heatmaps show average expression levels of the gene clusters.

(C) qRT-PCR of marker genes in cells differentiated in AP after various periods of the naive-to-primed transition. The insets show RNA-seq expression of the same markers in hsTE, hsAME-E, and cyAME-L3 of embryos.

(D) Validation of markers. Boxplots show gene expression in embryos by RNA-seq. Scatter plots show in vitro differentiated cells by qRT-PCR. Each dot in the scatter plots indicates an independent differentiation experiment.

(E) Flow cytometry of surface markers in the in vitro differentiated cells.