Figure 4.

Cyprinodon diabolis suffers from uniquely high mutation load. The mean proportion of homozygous ancestral, heterozygous and homozygous-derived genotypes in C. diabolis, C. nevadensis and C. salinus with respect to the C. brontotheroides genome at segregating sites across all populations in coding regions. Variants were categorized into mutation classes as synonymous (SYN), non-synonymous (NSYN) or loss-of-function (LOF) with Snpeff. Loss-of-function mutations are defined as those that encode a premature stop codon. Error bars correspond to the 95% confidence interval, spanning the mean ± two standard errors. (Online version in colour.)