Fig. 3.

An example of a pooled CRISPR screen. Cells expressing a Cas9 construct are transduced with a lentivirus sgRNA library in bulk. Following perturbation and selection, the cells are then exposed to a survival challenge (e.g. exposure to a compound). Alternatively, the population can be phenotypically characterized via microscopy or fluorescence‐activated cell sorting (FACS) or analyzing the growth advantage in the population. Finally, sgRNA abundance can be determined by next‐generation sequencing. Multi‐omic profiling such as single‐cell RNA‐sequencing (scRNA‐seq) can also be utilized. [Colour figure can be viewed at wileyonlinelibrary.com]